FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3940790
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
138520.3515031250079299No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT124220.31521598461222244No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG94180.2389876141585824No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC81000.20554254350016113No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG80160.2034109911971965No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT71780.18214621941285886No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT65380.16590582091408068No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG60680.15397927826654045No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT60320.15306575585098417No Hit
GCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCG56720.14393053169542147No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG55610.14111383758078966No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT51050.1295425536504102No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA51030.12949180240510152No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT50530.12822302127238447No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA50480.12809614315911275No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT50050.12700499138497612No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA48710.12360465794929443No Hit
GCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAAT48640.12342702859071404No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA48550.12319864798682498No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG47740.12114322255182336No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA47550.12066108572139089No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA45040.11429180443515133No Hit
GGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTT44830.11375891635941017No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG44200.1121602521321867No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG44120.11195724715095197No Hit
GGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAGTGGGA44120.11195724715095197No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG43400.11013020231983942No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA43080.10931818239490051No Hit
GCCGATTGTAACTATTATGAGTCCTAGTTGACTTGAAGTGGAGAAGGCTA42430.10766876692236836No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT42400.10759264005440533No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA42330.10741501069582494No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT42200.10708512760131852No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG41750.10594322458187318No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA40320.10231451054230244No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA39990.10147711499470918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)