FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533571
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
156520.34524660582132716No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA132760.29283758873523763No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG88600.19543093071664697No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG83820.18488736583148252No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT66020.14562471835116292No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC60420.1332724247618489No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG59480.13119900405221402No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG58500.12903735267408406No Hit
GCTTCACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCA56510.12464787691645283No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA53970.11904522946701397No Hit
GTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTATTCTGCCT47830.10550182185301608No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT47580.10495038017492171No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT47530.10484009183930285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)