FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813002
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
124410.3262783497097562No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA116250.3048778888655186No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG73400.19249924337831453No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG72060.18898495201418725No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT54430.14274841712645311No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC51610.13535266962881215No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG51110.13404136688100346No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG47600.12483602159138651No Hit
GCTTCACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCA47130.12360339700844636No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA46280.12137418233717161No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT41070.10771040770500515No Hit
GTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTATTCTGCCT40740.10684494789145141No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT40450.10608439229772236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)