FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008020484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008020484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3934701
Sequences flagged as poor quality0
Sequence length0-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
129610.32940241202571685No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA115070.2924491594151627No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG72600.18451211413522908No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG71230.18103027396490864No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT58560.14882960611238313No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC52990.13467351140531392No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG51270.13030215002359774No Hit
GCTTCACCGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCA48900.1242788206778609No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA47400.12046658691473634No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG46710.11871295938369902No Hit
CAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGAT40670.10336236476418412No Hit
CTTAATATGACTAGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTT40130.10198996060945928No Hit
GTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTATTCTGCCT39520.10043965221245528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)