FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008025308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008025308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38388832
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTATCGGTGTAGATCT2199210.5728775493872801Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCTATCGGTGTAGATCTC842810.2195456220184037Illumina Single End PCR Primer 1 (96% over 31bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT605510.15773076919870863No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG417690.1088050816445783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)