FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008025328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008025328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52525157
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGCTGTGTGTAGATCT4486550.8541716495964019Illumina Single End PCR Primer 1 (97% over 35bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAGCTGTGTGTAGATCTC704630.13415095551261275Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)