FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008025423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008025423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75389194
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTT1152140.15282561583030058No Hit
CCCCCGCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCG1030320.13666680134556156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGGTATATCTCGTAT974000.12919623467522415TruSeq Adapter, Index 12 (97% over 37bp)
CCCCGCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCGG926650.12291549369794297No Hit
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT845370.11213410770779696No Hit
GCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGA825510.10949977791246845No Hit
CCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCA818600.1085832009293003No Hit
CCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG802680.1064714924528839No Hit
GTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCA764480.10140445326952295No Hit
CTCCCGGCGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTA756970.10040828928347477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)