FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008025425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008025425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75962753
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTT1173100.1544309485465857No Hit
CCCCCGCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCG1049460.1381545505597987No Hit
CCCCGCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCGG953350.12550229715871408No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGGTATATCTCGTAT947970.1247940553181373TruSeq Adapter, Index 12 (97% over 37bp)
GTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGT863190.11363332237313727No Hit
GCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGA843760.11107548985224378No Hit
CCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCA840730.11067661015392637No Hit
CCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG820480.10801082999190405No Hit
CTCCCGGCGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTA780040.10268716827574692No Hit
GTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCA769820.10134177206558061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)