FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008043467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008043467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11599147
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG197339017.013233817969546TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT997970.8603822332797403TruSeq Adapter, Index 21 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAAACTCGTATG354160.30533279731690616TruSeq Adapter, Index 21 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAACCTCGTATG121020.10433525844615987TruSeq Adapter, Index 21 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAAGCTCGTATG116450.10039531355193619TruSeq Adapter, Index 21 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)