FastQCFastQC Report
Wed 31 May 2023
EGAF00008045624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170724
Sequences flagged as poor quality0
Sequence length40-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC8910.5218949884023336No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTG7600.44516295307045295TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC4630.2711979569363417TruSeq Adapter, Index 6 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTG4000.23429629108971203RNA PCR Primer, Index 6 (95% over 24bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTT3100.18157962559452684TruSeq Adapter, Index 6 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.16459314449052273No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT2140.12534851573299596No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGA2000.11714814554485602RNA PCR Primer, Index 6 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.10953351608444038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)