FastQCFastQC Report
Wed 31 May 2023
EGAF00008045648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674202
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT7630.11317083010729723No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCCTGTCTCTTATAC7440.11035268361707619No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCTGTCTCTTATAC7420.11005603661810555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.10723789012788452No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCTGTCTCTTATACAC7190.10664459612994324No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7070.10486471413611945No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCT7020.10412309663869286No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGG7000.10382644963972222No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6900.10234321464486905No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGT6770.10041500915155992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)