FastQCFastQC Report
Wed 31 May 2023
EGAF00008045656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68318
Sequences flagged as poor quality0
Sequence length41-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1273456482918118No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC720.10538950203460289No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG700.10246201586697502TruSeq Adapter, Index 5 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)