FastQCFastQC Report
Wed 31 May 2023
EGAF00008045657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411438
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG10200.24791098537325185RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC6430.15628114077941269No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC4770.11593484315984424RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.10912944356136284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)