FastQCFastQC Report
Wed 31 May 2023
EGAF00008045659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405479
Sequences flagged as poor quality0
Sequence length41-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG5420.13366906794186628TruSeq Adapter, Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.13046298328643408No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC5280.13021636138986237No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT4150.10234808707725924No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4100.1011149775944007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)