FastQCFastQC Report
Wed 31 May 2023
EGAF00008045665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502836
Sequences flagged as poor quality0
Sequence length38-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC100990.22428087543050648No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT76620.17015942841355983No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC75540.1677609399942614No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCC56910.12638701476136374No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC56780.12609830782200374No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG54320.12063508420026844No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT52610.1168374775363793No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG51620.1146388631520224No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT49700.11037488373993636No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGG48090.10679935933709332No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT47940.10646623594552411No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC47540.10557790690133952No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG46260.1027352539599488No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT46220.10264642105553033No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG45840.10180250846355497No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTC45500.10104742877599807No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)