FastQCFastQC Report
Wed 31 May 2023
EGAF00008045700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388174
Sequences flagged as poor quality0
Sequence length39-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG5380.1385976392030378Illumina PCR Primer Index 10 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.11850355768289478No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC4470.11515454409620428No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC4260.10974459907155039No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC4240.10922936621205953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)