FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008045721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469520
Sequences flagged as poor quality0
Sequence length35-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC25340.5397001192707446No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTG25300.5388481853808145TruSeq Adapter, Index 5 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC13250.28220310103935936Illumina Paired End PCR Primer 2 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTG11680.2487646958596013Illumina Paired End PCR Primer 2 (96% over 25bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8320.17720224910546942No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC8230.1752853978531266No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTT8180.17422048049071392Illumina Paired End PCR Primer 2 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.1552649514397683No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT6350.13524450502640997No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCTGCTTGA6130.1305588686317942Illumina Paired End PCR Primer 2 (96% over 29bp)
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG5450.11607599250298177No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCC4860.10350996762651218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.10223206679161698No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT4780.1018060998466519No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)