FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008045750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53130
Sequences flagged as poor quality0
Sequence length41-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC7151.3457556935817805No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG7091.334462638810465Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC4170.784867306606437Illumina PCR Primer Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG3310.6230001882175795Illumina PCR Primer Index 7 (95% over 23bp)
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTT3070.5778279691323169Illumina PCR Primer Index 7 (96% over 26bp)
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA2080.39149256540560884Illumina PCR Primer Index 7 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.3105590062111801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.15622059100319968No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGC780.14680971202710333RNA PCR Primer, Index 41 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.13175230566534915No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGT560.10540184453227931No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCT540.10163749294184077No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)