FastQCFastQC Report
Wed 31 May 2023
EGAF00008045754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305480
Sequences flagged as poor quality0
Sequence length41-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC20520.671729736807647No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG20390.6674741390598402RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC11480.3758020164986251RNA PCR Primer, Index 17 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG8600.2815241587010606RNA PCR Primer, Index 17 (95% over 24bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT6790.2222731439046746RNA PCR Primer, Index 17 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.20394133822181484No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA5020.16433154379992143RNA PCR Primer, Index 17 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.1273405787612937No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC3880.1270132250883855No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3730.12210291999476233No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)