FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008045757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181457
Sequences flagged as poor quality0
Sequence length36-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG6620.36482472431485147RNA PCR Primer, Index 27 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC4890.2694853326132362RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC3210.17690141466022252RNA PCR Primer, Index 27 (95% over 24bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3160.17414594091162094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.1537554351719691No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG2770.15265324567252847RNA PCR Primer, Index 27 (96% over 26bp)
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATC2740.15099996142336752No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT2240.12344522393735154No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATC2170.11958756068930931No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT2140.11793427644014837RNA PCR Primer, Index 27 (96% over 29bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCC1960.1080145709451826No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT1910.10525909719658101No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)