FastQCFastQC Report
Wed 31 May 2023
EGAF00008045799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132095
Sequences flagged as poor quality0
Sequence length40-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.19607100950073808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.13929369014724252No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1710.12945228812596996No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1640.12415307165297702TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.12415307165297702No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGT1610.1218819788788372No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCT1580.11961088610469738No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACCTGTCTCTTATAC1460.11052651500813807No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAATC1370.10371323668571862No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACCTGTCTCTTATAC1350.10219917483629205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)