FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008045801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008045801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182702
Sequences flagged as poor quality0
Sequence length35-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.22003043206970915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.12424603999956212No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCT2120.11603594925069238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.11494127048417642No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC2050.11220457356788649RNA PCR Primer, Index 29 (95% over 21bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGT2050.11220457356788649No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1990.10892053726833861No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG1950.10673117973530666RNA PCR Primer, Index 29 (96% over 30bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACCTGTCTCTTATAC1890.10344714343575878No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGG1850.10125778590272685No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACCTGTCTCTTATAC1830.10016310713621088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)