FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008046667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008046667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603751
Sequences flagged as poor quality0
Sequence length40-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC21600.35776338258652984No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG20520.3398752134572034RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT10430.1727533370545142RNA PCR Primer, Index 47 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC9950.1648030396637024RNA PCR Primer, Index 47 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTG8720.14443040259974724RNA PCR Primer, Index 47 (96% over 25bp)
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTGA6330.10484454684133028RNA PCR Primer, Index 47 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.10467891564568838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)