FastQCFastQC Report
Fri 2 Jun 2023
EGAF00008046684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008046684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333283
Sequences flagged as poor quality0
Sequence length40-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG8010.24033629077990776RNA PCR Primer, Index 47 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC6770.20313067273158247No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTT4150.12451880233915322RNA PCR Primer, Index 47 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC3780.11341712598602388RNA PCR Primer, Index 47 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)