FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008054927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008054927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24680259
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCTCA385580.15623012708253994No Hit
GCTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAG288410.11685857915834676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTAT287350.11642908609670588TruSeq Adapter, Index 9 (97% over 36bp)
CCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACACC285670.11574838011221844No Hit
GTCTGGGTTATTTCTTTGATAAGAACTGAATGTGGCCGGGCGCAGTGGCT264470.10715851887940074No Hit
CCCCTGTAGCCATCCATTCCCAATCTTTTCAGAGTTCTGGCCAACTCACA262940.10653859021495682No Hit
CTCCTGCTTCTGTAAAAATATTATTTGTTTGAAGGGGAAATAATGGTAGT251660.10196813574768401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)