FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008054954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008054954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26075488
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTAT3736471.4329434601569107TruSeq Adapter, Index 6 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCGT915500.351096017838669TruSeq Adapter, Index 6 (96% over 32bp)
AAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCGTC685350.2628330484169654TruSeq Adapter, Index 6 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCGTCTTC566400.217215493723454TruSeq Adapter, Index 6 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCGTCT560630.2150026875815325TruSeq Adapter, Index 6 (96% over 30bp)
GAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCGTCTT400590.15362703854286447TruSeq Adapter, Index 6 (96% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATG382350.14663196332126172TruSeq Adapter, Index 6 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGACAGTGCATCTCGTATGCCG355620.1363809567053932TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)