FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3616786
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGAGTTAAGTGTATTAAAAAGAAGAAATAGTCGTAAGATGGCAGTAT195600.540811648795367No Hit
CTCCAGGTGTTTCTTTGCTGCCATGTAACCCATTGTTGAGTTGTCTCTTA123210.3406615707979405No Hit
CCTGAGTTAAGTGTATTAAAAAGAAGAAATAGTCGTAAGATGGCAGTATA122280.33809022706900543No Hit
CTCCGCTTTGTACTTCTCCGCCTCCTGCACCATGCGCTCGATCTCCTCCT90560.2503880517122108No Hit
CTCAAATTCCTTCAGCTGTTGGACACAGTACTCATCAATGGGCTCAATCA78740.2177071023831656No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT78440.2168776366641543No Hit
CTCATTAGTAATATCGTCGGGATTTCTGGTCCAGATGGGCTTTGTTTTGT73580.20344029201617128No Hit
CTCCAATATGTCTTTCATGATTTTGCAGAGGTTCTCAAACTTTGTTTTTT73500.20321910115776826No Hit
GTCGTCATCTCCTTCAAGGGGTGGCATTTCTTCAGTTACAGCAGCACTGGTATCATCAGCAGTAGGGTCATCTTC70520.194979741682256No Hit
CTCCGTCGTTGATCACCTGGAAAGGCCAGTGCTTCATGTCCGACTGCACC70240.19420557367784547No Hit
CCTGCTTCTTTTTCTCTTCTTCATCCTCTGGAAGTTCCAGGCCTTCTTTG65850.18206772532298013No Hit
CTGTGATATAATAGATATGTTTCTGGTTCTCCTTCATTCTGGTGCAGTAG65640.18148709931967222No Hit
CTCCTCTTGGGTGATGTCATCAGGGTTTCTGGTCCAAATAGGCTTGGTCT59360.1641236169350357No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT59260.16384712836203194No Hit
CCCCTGCTGAGGACTCCCAAGCGTACTGCTCATCATCGTTATGTTTGGTGATCACAGTTACTTTCTCAGCAACCA58030.1604463189140856No Hit
CCAGGTGTTTCTTTGCTGCCATGTAACCCATTGTTGAGTTGTCTCTTAGG57690.1595062577658728No Hit
GGATGATGTCAATTTTCAGCTCTTTACCACTGTCCAACTTCGAAGGGTCT55080.15228990601047449No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT54310.15016094399834548No Hit
GTGGCATTTCTTCAGTTACAGCAGCACTGGTATCATCAGCAGTAGGGTCA52490.14512885196967693No Hit
GGCATTTCTTCAGTTACAGCAGCACTGGTATCATCAGCAGTAGGGTCATCTTCATCAATACCCAGACCAAGTTTG51730.1430275388148483No Hit
CTCCTCATTAGTAATATCGTCGGGATTTCTGGTCCAGATGGGCTTTGTTT50790.14042854622861292No Hit
CTCGGGTGTTTTGGGGAGGGGGAGGCCTTCTCCAGGAACTTTTCGCCGCA48500.13409695790682666No Hit
CTCTTCTTCATCCTCTGGAAGTTCCAGGCCTTCTTTGGTGACTGACACTA47940.13254862189800556No Hit
GTCAGGGTTTATCTCCAGGTGTTTCTTTGCTGCCATGTAACCCATTGTTG47680.1318297516081958No Hit
CTCTAATGAAGTTCAGATATTCAGGGATTAGCTCCTCACAGTTATCCATG47310.13080674388808183No Hit
CTCCGTACTCCTCATTAGTAATATCGTCGGGATTTCTGGTCCAGATGGGC47270.13069614845888033No Hit
CCTGTGTCTACCAAAGTCAGGGTACGTTCCTGAGGGTTGGGGATGATGTCAATTTTCAGCTCTTTACCACTGTCC45850.12677001072222688No Hit
CTGGAAGTTCCAGGCCTTCTTTGGTGACTGACACTAAAGTCTTCCCCTCAAATTCCTTCAGCTGTTGGACACAGT45080.12464104871009786No Hit
CTTCCTCTTGTGCTCAAACTCGTCCTTCTCGGCCAAGGTGTTGGCGTCCA42130.11648463580648674No Hit
CAGGTGTTTCTTTGCTGCCATGTAACCCATTGTTGAGTTGTCTCTTAGGG40860.11297323092933892No Hit
GCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTAA38670.10691813118055644No Hit
CCCCCTTGTAGCTCACCTGCACCTTGGGCTTGTCTCCGTCGTTGATCACCTGGAAAGGCCAGTGCTTCATGTCCG37890.10476152031112707No Hit
GTCTGCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCT37490.10365556601911198No Hit
CTCTTCTTGATCGATGTACTTTTCCTTAATCTTCTTCTTCTTCTTCTTGTCACCATCCTTCTTTTCTTCTTCCTC37390.10337907744610822No Hit
GGGGCTTGTCCTTTAAAACAAAGACGATATCAGCTGGAATGTTGTTGGAG37230.10293669572930221No Hit
CTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTA36850.10188603915188789No Hit
CTCTGAGTGAAGTGTACTATCCCATCACTGAAGCCCACAGGAACAAGTCCTACAATTTTAAAAAGGCTCGATGGA36590.1011671688620781No Hit
CTCCGAGTCTACCACCCCTCTAATGAAGTTCAGATATTCAGGGATTAGCT36370.10055889400146982No Hit
CTTCGTTTCGAATGCTCTTTCCGTCGGGGTTTAGCCGCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGT36320.10042064971496793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)