FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127810
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT43300.2034956128601708No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT43090.2025086826361376No Hit
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA42010.19743304148396706No Hit
GGCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC35350.1661332543789154No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCC34960.16430038396285382No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT33470.15729787903995188No Hit
GGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCTTCCACA32680.1535851415304938No Hit
GCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC29390.1381232346873076No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT29220.1373242911726141No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT26700.12548112848421616No Hit
CCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTT25650.12054647736405037No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT24870.1168807365319272No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTG24820.11664575314525263No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT21440.10076087620605222No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT21320.10019691607803327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)