FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175376
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT131850.4152264172809771No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT113200.3564932152916694No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT97080.30572757367946346No Hit
GCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTAA89610.28220280055023406No Hit
CCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTT67290.21191191216410277No Hit
CTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGAC63650.2004487027677982No Hit
GTCTGCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCT61490.19364635873043065No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTG57610.18142733332997416No Hit
CCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAAC52200.16438998090304896No Hit
CTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACT51710.16284685656123873No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT50680.15960314621008662No Hit
CTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTA50640.1594771768760613No Hit
CTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCTATTCAC50370.15862688387139035No Hit
GTCTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAG49380.15550914285426357No Hit
CTCCGTCATGTTTTAGAAACCTTTTATCTTTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTAA48430.1525173711711621No Hit
CCCCATTATATTAGAAAATAAATAAAAGGTTACCATAAGTAAGTTCCAGA48090.15144663183194684No Hit
CTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA47970.15106872382987085No Hit
GCCTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTTATTCCAGGATTAATGTAGTGT46100.14517965746418693No Hit
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAGGAGAAAGTGCCATGGTTGATATTTA44090.13884969842941436No Hit
CCTCCGTCATGTTTTAGAAACCTTTTATCTTTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTA44050.13872372909538902No Hit
CTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACACCCTCATCTTCTCAAGCTTTACCTTCT43200.13604688074735088No Hit
CTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCTGGGTCAGCTGTCAATTAATGCTAGTCCTC43020.1354800187442369No Hit
GCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC42510.13387390973541402No Hit
CCCAGCATTACAGTTCTTGAACATGTTATTTCTCCACTTACTGGTTTAAG42340.1333385400658064No Hit
GTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACA39620.1247726253520843No Hit
CCTGTCTGCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTGTGTAGCACCTGGGTCAGCTGTCAATTAATGCT39220.12351293201183106No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT38440.1210565299983372No Hit
GGCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC36310.11434866296148864No Hit
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA36070.1135928469573367No Hit
CATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTGA35190.11082152160877955No Hit
CTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACTTCAACATTTGGCCTACTCAAGCTCTTCTGA35050.11038062893969092No Hit
CCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACA33780.10638110258438685No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA33420.10524737857815893No Hit
CCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACC32590.10263351489713343No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA32570.10257053023012078No Hit
CCAGCATTACAGTTCTTGAACATGTTATTTCTCCACTTACTGGTTTAAGTTGGTTTTAGTCACTGGATAAGTATT31900.10046054388519658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)