FastQCFastQC Report
Thu 9 May 2024
EGAF00008060272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577400
Total Bases270 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGACTTTCTGGAAATAAAATGTGTAATTACCTTTTACTCTGATCATAA140990.3941130429921172No Hit
CCCTGACTTTCTGGAAATAAAATGTGTAATTACCTTTTACTCTGATCATA136740.38223290658019793No Hit
CTGACTTTCTGGAAATAAAATGTGTAATTACCTTTTACTCTGATCATAAT128660.35964667076647844No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT124320.3475149549952479No Hit
CTCAACACTCAGCCTTTATCACTCAGCTGGATTTTTTCCTTCAACAATCA107300.2999385028232795No Hit
CGTAACTTTAAATTGGTAGCTTTCATTTGCTTAAAATTTTTTGGCATATG100500.28093028456420865No Hit
CCAGAAATGAACAAAAAGCATTTTACCTAAAAATACACCAGCAAAATGTA88650.24780566892156314No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT86830.2427181752110471No Hit
CTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATTAATTCGGGGC85600.23927992396712694No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA82040.22932856264326046No Hit
CCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTT78610.219740593727288No Hit
CTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACT75820.2119416335886398No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT74520.2083077095096998No Hit
CTCTGAGTGAAGTGTACTATCCCATCACTGAAGCCCACAGGAACAAGTCC72900.2037792810420976No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA71160.19891541342874713No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCT71090.1987197405937273No Hit
CTCCAGTCTACAAGTTACATGTTCCCACCCAGCATTACAGTTCTTGAACA69750.1949740034662045No Hit
CCCTAACATGCAATACTGCAGATGCAAGTTAAACTTATCTGTTAACAGCT67880.18974674344496004No Hit
CTCAATTTTCTTTTAAATACATTTTTTACTCCATGAAGAAGCTTCATCTC65800.183932464918656No Hit
CCCAACTCATCTCTCATTTATTTCGGCTTCTTTTATTCCAGGATTAATGT65020.181752110471292No Hit
CTTCAAATTATTTTAATCACCTACAACTTTTAAACTAAACTTTAAGCTGT60960.17040308604014087No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGG57070.15952926706546655No Hit
CCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAAC57060.15950131380332086No Hit
CTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACTTCAAC56070.1567339408508973No Hit
CGCTTTTATTCTGCTTTTGTAAAAGCAGTATTTTGAGATGGACATTGCCT55740.15581148320008947No Hit
CTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATTATTTTAATCAC55480.15508469838430144No Hit
ATTTTTTCTTACTGGGTCTGGCTTCTCTGGCCCTTCGCATACGTGTGTCT55240.15441382009280483No Hit
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAG49470.1382847878347403No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT49330.13789344216470062No Hit
CTCCGTCATGTTTTAGAAACCTTTTATCTTTTCCTTCCTCATGCTACTCT49260.13769776932968078No Hit
CTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGG48990.13694303125174706No Hit
CCAGGATTAATGTAGTGTAACATTTTCATTTCTTTTCGCTTTTATTCTGC48730.1362162464359591No Hit
CATAATTGTAGTTAATGTCAGCCCAGTAGGCCAGACCAACCCCCAGTTCA47000.13138033208475428No Hit
CTCAGGGTTATGCTTATTCCCCAATGGAGGTATGACATATAATCTTTTCT46500.12998266897746968No Hit
CTCATTTTGTCCACTGGTGAATTCAACTGGAAGCTCCTTCTATAGTCTGA45370.12682395035500643No Hit
CTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAG45290.1266003242578409No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT44530.12447587633476827No Hit
CCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACC44460.12428020349974842No Hit
CTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACACC43710.12218370883882149No Hit
CACCAGTCCTTTTAGTAGCTTTTTGATGTGATTTTTAACCAACTTCCCCT43700.1221557555766758No Hit
TCTTGATCTTGAATTACTTCCGTTACGAAAGTCCTTCACATTTTTCAAAC42680.11930452283781517No Hit
CCCACACATAAACAATGCACTAAAGATCACTGAACTGTTTAAACATTTCC42110.1177111868955107No Hit
CGCCTTCAAATTATTTTAATCACCTACAACTTTTAAACTAAACTTTAAGC41680.11650919662324594No Hit
CTCTGATCATAATCTCCCACCTGTCTAAGAGGTTATTTATTCCTTATTTA40640.11360205736009393No Hit
CCCAACTAAACATACAAACTGCTTACAGATTTGCTGAAATTGTCTCAATT40200.11237211382568346No Hit
CCCACATTTAAAATGGAATGTTTGGTTTATTTAAAGTAAATAGGCTATTT39710.11100240398054452No Hit
CCCCAACTAAACATACAAACTGCTTACAGATTTGCTGAAATTGTCTCAAT39030.10910158215463744No Hit
CCCAGTTTGTCAATAGTACTATAGCATCTGTGGAAAATCTTAGAAAAAAA39020.10907362889249175No Hit
CCCAAATTAATGCACTGGTACACCCAGTGGCTCATATTTAACTTCCCCCA37800.10566333091071728No Hit
CTCCTTCTCTAACCCAGTTTGTCAATAGTACTATAGCATCTGTGGAAAAT37420.10460110694918098No Hit
CCCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAA37290.10423771454128695No Hit
CCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGG36540.10214121988036003No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCC36440.10186168725890311No Hit
GCCAGTTTAATTTCTTGAAGACTGTTGCTTGTTTGGAATGTTTCTTGTCA36300.10147034158886341No Hit
CTATTTTTTCTTACTGGGTCTGGCTTCTCTGGCCCTTCGCATACGTGTGT36180.1011349024431151No Hit
CTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA36070.10082741655951251No Hit
CTGGAATCCTGTCTGCTGCTGTCTTCCTAGATTCACTGAATCCACTTCTG35930.1004360708894728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)