FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577400
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAAAATCAGTGACAAGAAACATTCCAAACAAGCAACAGTCTTCAAGAAATTAAACTGGCAAGTGGAAATGTTT61340.1714653100016772No Hit
CTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATGTAACTTTAA55760.15586738972438083No Hit
TGGCAATAGAGGCCCTCTAAATAAGGAATAAATAACCTCTTAGACAGGTGGGAGATTATGATCAGAGTAAAAGGT54390.15203779281042099No Hit
CTTCTGAGTCATAACCAGCCTGGCAGTATGATGGCCTAGATGCAGAGAAAACAGCTCCTTGGTGAATTGATAAGT53130.14851568178006375No Hit
CTAGAATCCTAAAGGCAAATGACTCAAGGTGTAACAGAAAACAAGAAAATCCAATATCAGGATAATCAGACCACC51260.14328842175881926No Hit
GGAAATTATTGGTAACCAATTTATTTTAAAAGCCCATCAATTTAATTTCTGGTGGTGCAGAAGTTAGAAGGTAA47840.13372840610499245No Hit
GGAAAACCAAATGAATTTGATAGCCAAATTGAGACAATTTCAGCAAATCT45650.12760664169508582No Hit
CGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAA45570.12738301559792028No Hit
CCTTAATCAGACTTTAAAAGTGCTTAACCCCTTAAACTTGTTATTTTTTACTTGAAGCATTTTGGGATGGTCTTA39590.11066696483479621No Hit
GGGGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGAC39240.109688600659697No Hit
CGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGA37250.10412590149270419No Hit
CCATAATCGGTTTCAAGGTAACGATGGTGTCGAGGTCTTTGGTGGGTTGA36770.10278414490971095No Hit
TGAAGAGGCAATGTCCATCTCAAAATACTGCTTTTACAAAAGCAGAATAAAAGCGAAAAGAAATGAAAATGTTAC36040.10074355677307542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)