FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502551
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT91250.2605244006439878No Hit
CCCCATTATATTAGAAAATAAATAAAAGGTTACCATAAGTAAGTTCCAGA72860.20801981184570903No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT63960.18260976071440502No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA59580.1701045894834936No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT58860.16804894489759037No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT55640.15885564549952305No Hit
CCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAAC52040.14857742257000683No Hit
CTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGAC51630.14740684718081193No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT46180.1318467596902943No Hit
CTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACT45260.12922010271941795No Hit
CCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACC41840.11945579093637752No Hit
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAGGAGAAAGTGCCATGGTTGATATTTA38870.11097625701952663No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTG38480.10986278286882903No Hit
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA38420.1096914791533371No Hit
GTCCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCA37000.10563729122002792No Hit
CCTCCGTCATGTTTTAGAAACCTTTTATCTTTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTA36060.10295353301065424No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA35040.10004136984729131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)