FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136303
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCATTATATTAGAAAATAAATAAAAGGTTACCATAAGTAAGTTCCAGA111060.3541111939758371No Hit
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA94420.3010550957608369No Hit
CCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAAC93120.29691008808778996No Hit
CTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGAC92610.2952839696929793No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT90950.2899911137412425No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT89230.28450694974305735No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA79710.25415273970659086No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT77870.2482859596155091No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT71940.22937834769153365No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT69480.2215347177871526No Hit
CTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACT57940.18473980352025934No Hit
CTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTT55150.17584397936041257No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA49310.1572233295061096No Hit
CCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAG46130.1470840030443487No Hit
CCCCCATTATATTAGAAAATAAATAAAAGGTTACCATAAGTAAGTTCCAG43060.13729540800107642No Hit
GCCTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTTATTCCAGGATTAATGTAGTGT41560.13251270683986846No Hit
CTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCAAA40910.13044020300334502No Hit
CCCCAACTAAACATACAAACTGCTTACAGATTTGCTGAAATTGTCTCAAT40380.1287503152597182No Hit
CCTGCCTTAAAGTTACATTCGTTCTTCCGCTCAAATCCTGATCTGGTCCA38150.12164003286672237No Hit
GCTCCTTCCTGGAATCCTTTTTCCTAGCTTCACCACCAAATCGTTAGCGCTCCTTCCTTCTTCCTAGCTTCCTTC37740.1203327612159922No Hit
CCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACA37680.12014145316954387No Hit
GCTCGCTTGCTCCTCAGTCCTAGCTTCATCAAACACTGGTTCCTGGAATC36970.11787764128657213No Hit
GTCCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCA36740.1171442937751869No Hit
CCCCTGTCCGCAGCGTCACTATGTCCTCATAGGTGGCTGTCTGGTCAATG36170.11532686733392787No Hit
CCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACC35330.11264855468365143No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT34410.10971516463811053No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTG33620.10719627535987433No Hit
GTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCT33460.1066861205693455No Hit
CCTCCGTCATGTTTTAGAAACCTTTTATCTTTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTA32790.10454984738400594No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT31980.10196718875695365No Hit
CCCCTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTA31790.10136137994320064No Hit
GCCTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCA31780.10132949526879258No Hit
GCCCCTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTT31490.10040483971095904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)