FastQCFastQC Report
Wed 8 May 2024
EGAF00008060408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761626
Total Bases283.5 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAG99280.26392841818936813No Hit
CTCTGAGTGAAGTGTACTATCCCATCACTGAAGCCCACAGGAACAAGTCC76360.2029973208394455No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT73360.19502204631720432No Hit
GGGGTTAAGCACTTTTAAAGTCTGATTAAGGGGGTGGGGGGAAAAAAGAG68270.18149066387780177No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT67090.17835372256572024No Hit
GGGTTAAGCACTTTTAAAGTCTGATTAAGGGGGTGGGGGGAAAAAAGAGT64310.17096330150844344No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT57560.15301893383340076No Hit
GTCTGATTAAGGGGGTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTC55520.14759574715827675No Hit
GCTATTTTTTCTTACTGGGTCTGGCTTCTCTGGCCCTTCGCATACGTGTG55170.1466652984640153No Hit
GTCTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAG55060.1463728717315331No Hit
CTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATTAATTCGGGGC45740.12159635221577052No Hit
CTCAACACTCAGCCTTTATCACTCAGCTGGATTTTTTCCTTCAACAATCA45720.12154318371895559No Hit
CTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATTATTTTAATCAC45130.11997471306291481No Hit
CCTATCTTCACCACGAACTGCTGCTTGCTCGCTTGCTCCTCAGTCCTAGC44950.11949619659158035No Hit
CTTCAAATTATTTTAATCACCTACAACTTTTAAACTAAACTTTAAGCTGT44140.11734287247057522No Hit
GGCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACC42230.11226528102474835No Hit
GTCCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCA41940.1114943378209317No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT40710.10822447526681281No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA40090.10657625186554964No Hit
GCTCGCTTGCTCCTCAGTCCTAGCTTCATCAAACACTGGTTCCTGGAATC38770.1030671310757635No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA38300.10181767140061239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)