FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761626
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAGGAGAAAGTGCCATGGTTGATATTTA96350.2561392334059792No Hit
CTCTGAGTGAAGTGTACTATCCCATCACTGAAGCCCACAGGAACAAGTCCTACAATTTTAAAAAGGCTCGATGGA73890.19643101148280026No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT66850.17771570060394096No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT65410.1738875688332652No Hit
GGGGTTAAGCACTTTTAAAGTCTGATTAAGGGGGTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGG65240.17343563661033817No Hit
GGGTTAAGCACTTTTAAAGTCTGATTAAGGGGGTGGGGGGAAAAAAGAGT63060.1676402704575096No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT55590.14778183689712907No Hit
GTCTGATTAAGGGGGTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTC54680.14536267029204925No Hit
GTCTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAG54550.14501707506275213No Hit
GCTATTTTTTCTTACTGGGTCTGGCTTCTCTGGCCCTTCGCATACGTGTGTCTGCTGAGTGTTCCTGCATGTA52950.1407635953175568No Hit
CTCAACACTCAGCCTTTATCACTCAGCTGGATTTTTTCCTTCAACAATCA45220.12021397129858204No Hit
CTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATTAATTCGGGGC44950.11949619659158035No Hit
CTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATTATTTTAATCACCTACAACTTTTAAACTAAACTTTA44130.11731628822216776No Hit
CCTATCTTCACCACGAACTGCTGCTTGCTCGCTTGCTCCTCAGTCCTAGC43980.1169175244960557No Hit
CTTCAAATTATTTTAATCACCTACAACTTTTAAACTAAACTTTAAGCTGTTTAAGTCACCTTCATTTTAATCTAA43100.11457811063619829No Hit
GTCCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCA41730.1109360686043748No Hit
GGCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC40830.10854348624770246No Hit
CCCCAGTTCAATACTTTCCTTCCCCAAAAGCTCTATACTTTGAAGGAAAA39890.10604456689740022No Hit
GCTCGCTTGCTCCTCAGTCCTAGCTTCATCAAACACTGGTTCCTGGAATC38430.1021632666299095No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA37790.1004618747318314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)