FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181281
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA40600.186129159883573No Hit
GGTCGGTGCTGGGTATGTAAGGAGCTTGATAACCACTGGCAGGTTTGCTT30760.1410180531531701No Hit
GGGGCTTTTAAACTTCTGTTTGCCAGCTCTGTCTTCAGTACCAGGCACTT30460.1396427145333407No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT30370.13923011294739193No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT29740.13634190184575026No Hit
GTCTGGCGCAGAGTAATTGTGTCAGCTTCAAAGAGCAGGACAGTTGAATCTTCAGGCTTAAGGGTCAAACTGCCC27030.12391800964662508No Hit
CTCAGGAATTTGAATGGTTTTGAGAGACCCAAATGTGGTGATGGTCTGGCGCAGAGTAATTGTGTCAGCTTCAA26550.1217174678548981No Hit
GTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCT23770.10897266331114606No Hit
GCCTCCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTTATTCCAGGATTAATGTAGTGT22130.10145414552274559No Hit
CAGGAATTTGAATGGTTTTGAGAGACCCAAATGTGGTGATGGTCTGGCGCAGAGTAATTGTGTCAGCTTCAAAG22020.10094985469547482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)