FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151940
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTCCGCAGCGTCACTATGTCCTCATAGGTGGCTGTCTGGTCAATG81520.2586343648673516No Hit
GGGCCATCTGTTAAATATCCAGAAGGTTCTGTTAACCTTGCCAACTATCA80290.25473200631991727No Hit
CACCGCTACTACTATGTCCTATCATAACATTCCATACATACTTAAAACCAAGCAAAGGGTGGAGTTCCATCTTTA77680.24645139184121526No Hit
CTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCACTAATATGCACTGGCCCTGA71320.22627334276667704No Hit
CTCCTCTTCTTCGTCCTGGTTAATCTGGAAGTAACGTAATTCGTAACTCT67400.21383655780249622No Hit
CTCCACAGCTACTAAGTGCTGTCCACTAATATGCACTGGCCCTGAACCAC49550.15720476912631584No Hit
CTCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGT49250.15625297435864896No Hit
GCACCGTCCAATCATCATCTTCTGAGCAAGGAAGACCTTCTCTGCAGGAA44680.14175396739785656No Hit
CTCCGACTCTGTTTTGCTTTACTGACTGGTTCTTCATCAATTTCAGCTGC41210.1307448745851761No Hit
CTCTCCTTCTAGATGAGGTAGATGGTCTAGAAACCAAATGTGAAGATGAA40670.1290316440033757No Hit
GCCTGCTCATTTCAAACAGGAGCCAATGGTGCATCCAGCTCTCAAAGACC37290.11830808962099533No Hit
GCCATCTGTTAAATATCCAGAAGGTTCTGTTAACCTTGCCAACTATCATTGCAGACACATAATTTTTCTCCAGTC34660.10996402215778218No Hit
GTCCAGTACATCCAGAATGTCACAACAGATTAACTTTAGCTCAGTCTCAA34130.10828251806823733No Hit
CTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTA33740.10704518487027037No Hit
CTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAACTGGTGCTC33180.10526850130395883No Hit
ACCGCTACTACTATGTCCTATCATAACATTCCATACATACTTAAAACCAAGCAAAGGGTGGAGTTCCATCTTTAA32460.10298419386155827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)