FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583319
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA160180.6200550532086824No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT129430.5010221347034571No Hit
CTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGAC124510.481976867742621No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT105050.4066474175276069No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT90560.35055678373441296No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACAT69390.26860794195374244No Hit
GCCTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCA68950.26690470669708233No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT58600.22683996827337236No Hit
CTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCAAA57210.2214592932580142No Hit
GCTCCTTCCTGGAATCCTTTTTCCTAGCTTCACCACCAAATCGTTAGCGCTCCTTCCTTCTTCCTAGCTTCCTTC55900.21638829738023063No Hit
GCTCGCTTGCTCCTCAGTCCTAGCTTCATCAAACACTGGTTCCTGGAATC53700.20787212109692998No Hit
CCCCATTATATTAGAAAATAAATAAAAGGTTACCATAAGTAAGTTCCAGA51780.20043982179514028No Hit
GTGGGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCT50670.1961430237612931No Hit
GGCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC49880.19308494227774425No Hit
CCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGG46950.18174294386407563No Hit
GCTCGATGGAAAAATTTCTCAATCCTGAAATCCCCTAGGGAAGGGGTCAGGAGAAAGTGCCATGGTTGATATTTA44870.17369128628713681No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT44010.17036223555821017No Hit
CCCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAAC43830.1696654574986674No Hit
CCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACCTGTTTTCCTCATTTTGTCCACTGGTG39170.15162664773494872No Hit
GCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTAA38070.1473685595932984No Hit
CTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTTC37960.14694275077913335No Hit
CTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCAAATTG37750.1461298430430001No Hit
GTCTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAG37010.14326531102043533No Hit
GTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACA36720.14214272414672752No Hit
GCCTACTCAAGCTCTTCTGAAGTTCTCCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTC36610.1417169153325625No Hit
CCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATT36420.1409814273808229No Hit
TCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACT36390.14086529770423242No Hit
CTCCCCTTTAATAAAACTTTTAAGAAGTCACATTATTGGAAAACTTAACT36160.13997497018370553No Hit
GGGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCTTC35470.13730398762212487No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT35080.13579430182644883No Hit
CCCTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACA35050.1356781721498584No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA34930.13521365344349653No Hit
CTTCTAGCTTCAAGTATTCTTCTAAATTGGTTCTGGTCTACGTAAACACCCTCATCTTCTCAAGCTTTACCTTCT33640.13022007735010657No Hit
GTCCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCA32590.12615553866944038No Hit
CCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTC32400.12542005071770077No Hit
CCTGCCTTAAAGTTACATTCGTTCTTCCGCTCAAATCCTGATCTGGTCCA31170.12065873397749174No Hit
CCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTT30860.11945872731939028No Hit
GCCTGCTACCTTCATCACCAAATTGCACTCGCTCCTTCCTGGAATCCTTTTTCCTAGCTTCACCACCAAATCGTT30000.11612967659046367No Hit
CCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTCAG29880.11566515788410181No Hit
CCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAG29410.1138457929508512No Hit
CCCATAACTGATCTGACTTTGTATGTAAATACAGAAAAAGCTTGTTCACC29350.11361353359767028No Hit
CCCCAACTAAACATACAAACTGCTTACAGATTTGCTGAAATTGTCTCAAT29280.11334256435229255No Hit
CCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCT28860.11171674888002606No Hit
GGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCTTCCACA28440.11009093340775956No Hit
GGGGGAAAAAAGAGTAACTACCAGCCATTTCTCCAATGGACATCTCTTCC27970.10827156847450896No Hit
GTGGCGATGTGGCAGAGAAGTTGCTTGTGGGGAGACCTTGCTTTTAACAG27800.10761350030716299No Hit
GGGTGAAGGGTCTGTGCTAGATCAAAAGGCACGGGGTGGCGATGTGGCAG26790.10370380119528405No Hit
CTGCTGAGTGTTCCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTA26450.10238766486059213No Hit
GCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTC25880.10018120100537331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)