FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008060432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350295
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGA141200.6007756473123587No Hit
GTCCACCATATCTGGCACTGAGATAGACTCAACAGCCCCATCTTTCTTGG72110.30681254906299No Hit
CTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCT67970.289197739007231No Hit
GCCTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCA66660.283623970607945No Hit
CTCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGAC48880.20797389263900917No Hit
GCTCCTTCCTGGAATCCTTTTTCCTAGCTTCACCACCAAATCGTTAGCGCTCCTTCCTTCTTCCTAGCTTCCTTC42260.1798072156899453No Hit
CCTCCATCTCCTGCTCTGGTGTCTCCTCATCCTCAGGCAGCGTCTTAGCA40070.17048923645755107No Hit
GCCCCATCTTTCTTGGAGGCATCTTCACTTCCAGAGCCCCACTCCTCCTC39400.1676385304823437No Hit
GCCTGCTCATTTCAAACAGGAGCCAATGGTGCATCCAGCTCTCAAAGACC39380.16755343478159124No Hit
CCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTCAG39040.16610680786879944No Hit
CTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCAAATTG37570.15985227386349374No Hit
CTCCTCCGTGTGGTTGCCAAGCCAAGCCGCCTGCTACCTTCATCACCAAA34950.14870473706492165No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT34110.14513071763331836No Hit
CCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTC32300.13742955671522086No Hit
CCTGCCTTAAAGTTACATTCGTTCTTCCGCTCAAATCCTGATCTGGTCCA32120.13666369540844872No Hit
CACCATATCTGGCACTGAGATAGACTCAACAGCCCCATCTTTCTTGGAGG30860.1313026662610438No Hit
CTCTGAGTGAAGTGTACTATCCCATCACTGAAGCCCACAGGAACAAGTCCTACAATTTTAAAAAGGCTCGATGGA29440.12526087150761925No Hit
CTCCTGCTCTGGTGTCTCCTCATCCTCAGGCAGCGTCTTAGCACCCAAAG28950.12317602683918402No Hit
GTCTCCTCATCCTCAGGCAGCGTCTTAGCACCCAAAGGGGTCTCAAAGGTGGAAGTCTCAGACCTTAGATGAAG28700.12211233057977829No Hit
GTCGGGAATAGTCAGCAGGAGGGCAGGGATCCAGACGCCAACATAGACCA26880.11436862181130453No Hit
GGGAGGGATGGCAAGCAGAGGAGGGGAGGCAGCTGCCCAGGAGAGGGCAG26870.1143260739609283No Hit
CTCCAGTTCCAGTGACTCTTTCGGTGGAGCGTCAGTGGGCTTTTGCTCCA26650.11339002125265127No Hit
CCCCGCCTCAGTTACACATCCAAACTCTACATTTACAAAACGAATTCAGGGTGAGGAAGTAAAAACAGGTCATCT26620.11326237770152257No Hit
CTCCTCATAGTCCTGCAGCCGCAGCATCAACTCCTCCTTCTCGCGCATCA26200.11147536798572094No Hit
GGGGTGGTGAAAGGAGAAAGCAGCCTTCCAGTTAAAGATCAGCCCTCAGT25660.10917778406540457No Hit
GCCTGCTACCTTCATCACCAAATTGCACTCGCTCCTTCCTGGAATCCTTTTTCCTAGCTTCACCACCAAATCGTT25010.10641217379094964No Hit
CCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACTCTTTT24670.10496554687815784No Hit
TCCTTCCTTCTTCCTAGCTTCCTTCACCAAATCGCACTGGCTCCTGGACT23550.10020018763602016No Hit
CTGTGGCCTTGGCACACTGGCATGCTGGTCTAGGATCCTCTACGCACAAC23510.10002999623451524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)