FastQCFastQC Report
Wed 8 May 2024
EGAF00008060787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008060787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368538
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGTAT5880.15954935447633622Illumina Paired End PCR Primer 2 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)