FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22619
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG444219.638357133383437TruSeq Adapter, Index 2 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG5642.49347893363986No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA3321.4677925637738185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATCTCGTATGCCGT2301.0168442459878861TruSeq Adapter, Index 2 (97% over 39bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA2200.9726336265971086Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACCGATGTATCTCGTATG1660.7338962818869093TruSeq Adapter, Index 2 (97% over 49bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA940.4155798222733101Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC760.3360007073699103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC690.3050532737963659Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC650.2873690260400548Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA630.2785269021618993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG620.27410584022282153No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC540.2387373447101994No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG490.21663203501481057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT480.2122109730757328TruSeq Adapter, Index 2 (100% over 48bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450.19894778725849951No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC450.19894778725849951Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT440.19452672531942172No Hit
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG390.1724214156240329Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC390.1724214156240329Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT350.15473716786772182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC340.15031610592864406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA330.1458950439895663TruSeq Adapter, Index 2 (100% over 36bp)
AGATCGGAAGAGCACACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT330.1458950439895663Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC330.1458950439895663Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC270.1193686723550997Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG260.11494761041602193TruSeq Adapter, Index 2 (100% over 35bp)
AGATCGGAAGAGCACACGTCTTAACTCCAGTCACCGATGTATCTCGTATG250.11052654847694417TruSeq Adapter, Index 2 (97% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC250.11052654847694417No Hit
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCA250.11052654847694417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGTTCGGAAGATTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC240.1061054865378664Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC230.10168442459878864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC230.10168442459878864Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)