FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56255108
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1157142220.56954899100007No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC17590583.1269302691588465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC16541812.9404991987572044Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC16274432.8929692926729427Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG13339552.3712602240493434No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG13106252.3297884345009168TruSeq Adapter, Index 4 (100% over 49bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC12647842.2483007231983274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC10945371.945666871708788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC10605911.8853239069419259No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT7692031.3673478326625914No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC6012791.0688433839643505Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC5700451.0133213147506532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG5669991.007906695335115No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA5654001.0050642867844108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA4535050.8061579048075066Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT3033060.5391617059912142Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTC3016390.5361984195284097Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC2853020.5071575011463848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC2436530.4331215576014893Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC2210850.3930043117151246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC2162690.3844433113522776Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA2126940.3780883328852555No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT2110100.3750948269444261Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC2007540.35686359361357906Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1951920.3469764914503409Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1790930.31835864576066586No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC1781520.3166859087711644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC1775390.31559622994590997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG1707420.3035137715849732No Hit
TGAGGTAGTAGATTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1671460.29712146317450855Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1600130.2844417257184894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC1544450.27454395785712477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA1523760.27086606961984677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC1449950.25774548330793356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC1352270.24038172675803948Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1322560.23510042856908211Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT1269520.22567195142528212Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC1178100.2094209827132498Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC1170180.20801311056055566Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA1105150.19645327140781596TruSeq Adapter, Index 4 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC1058740.18820335390699097No Hit
TCACAGTGAACCGGTCTCTTTAGATCGGAAGAGCACACGTCTGAACTCCA1030160.18312292636608216Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1020050.18132575623177188Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACTGAAGCTCCTTGAGGTAGATCGGAAGAGCACACGTCTGAACTCC1013240.18011519949441748Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC1010330.17959791313528364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC1001720.1780673854541351No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC974830.17328737507712189Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA952800.16937128624835276Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC948190.16855180510896894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC931060.1655067482938616No Hit
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA923030.16407932236126896Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA911240.1619835126794175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA875100.15555920717457336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA874290.15541522024986604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG868250.15434153997180133No Hit
AACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT863780.15354694546137926Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC818260.14545523581609693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG804000.14292035489470575Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAG773800.1375519535043822Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC750990.13349721059996897Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA746370.13267595184423076Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACGTCTGAACTC729380.12965578165808517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC707010.12567925387326606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT703170.12499664919317192No Hit
ACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC700850.1245842421989484Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCA685030.12177205312626899Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATGTTGCTCGGTGAACCCCTAGATCGGAAGAGCACACGTCTGAACTCC654490.11634321277989547Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC653080.11609256887392341Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC646070.11484645980948077Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA643900.11446071706057341TruSeq Adapter, Index 2 (97% over 36bp)
TCAACTTAACTTGACCGCTCTGACCAAGATCGGAAGAGCACACGTCTGAA632690.11246800912727782Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC623200.11078105120694107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTC608450.1081590670841837Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTC607870.10805596533562783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG601020.10683829813285578No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT599400.10655032428344108No Hit
TCACAGTGAACCGGTCTCTTTTAGATCGGAAGAGCACACGTCTGAACTCC581520.10337194624175283Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA573460.10193918746009695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)