FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11031214
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG144074113.06058426570276TruSeq Adapter, Index 5 (100% over 49bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA3923023.55629035933851Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1698521.5397398690660882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC665450.6032427618573984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCC601960.5456878998086702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCA471560.4274778823074233Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC470770.4267617326615185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTGAAGATCGGAAGAGCACACGTCTGAACTC434380.393773523022942Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG406810.36878080689940385No Hit
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA368770.3342968416712793Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGTAGATCGGAAGAGCACACGTCTGAACTC356960.32359085772427226Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC349300.3166469257146131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA325230.2948270244779949Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT320580.2906117132710869Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG311160.2820723086325766TruSeq Adapter, Index 5 (97% over 49bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA290030.2629175719009712Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGTAGATCGGAAGAGCACACGTCTGAACTCC261800.23732655354161383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG256980.23295713418305547Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC253420.2297299281837883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC252020.22846080222902032Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT250700.22726419775738194No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA231830.21015819292418766TruSeq Adapter, Index 5 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC230610.2090522403064613No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC216810.19654228446660538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA183420.16627363044538887Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGTTCGTTCGGCTCGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG182150.16512235190070648Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC167560.1518962464149458Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164770.14936705969080102No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA150060.13603217198034595TruSeq Adapter, Index 5 (100% over 40bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG145430.1318349911442204Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA140050.12695792140375484TruSeq Adapter, Index 5 (100% over 36bp)
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC139920.12684007399366926TruSeq Adapter, Index 5 (100% over 35bp)
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC135780.12308708724171247Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT131670.1193612960459293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC125320.11360490332251735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGTTCGGAAGATTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC115840.10501110757165984Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT114010.10335217864507025TruSeq Adapter, Index 5 (100% over 48bp)
CAGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG112490.10197427046560786TruSeq Adapter, Index 5 (100% over 39bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC110390.10007058153345587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)