FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12342078
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG11224259.094295142195666TruSeq Adapter, Index 6 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG7697596.2368670818641725No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1854681.5027291190348984Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1031140.835467090711953No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC911560.7385790302086893No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA768210.6224316521091505Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTAAGTGGGAAACGATGTGGGAAGGCAGATCGGAAGAGCACACGTCTG620170.5024842656155634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT594720.48186375098261414No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC546910.4431263519805984Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC425200.34451248809155155Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA416250.33726087292593676Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC333760.2704244779525782Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA332930.26975198179755466Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG325110.2634159336863695No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG322160.2610257365088764Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC320600.2597617678319648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC315620.2557267909018238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC266100.21560388777319348Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC262940.21304354096611608No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC260550.21110707613418095Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC259310.21010238308330254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC251020.20338552389638115Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC239420.19398678245267936TruSeq Adapter, Index 6 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA177400.14373592518212897No Hit
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG165860.13438579791830843Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC162490.13165530148164678No Hit
AGTTAAGTGGGAAACGATGTGGGAAGGTAGATCGGAAGAGCACACGTCTG162000.13125828567928352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC156230.12658322204737324TruSeq Adapter, Index 6 (100% over 36bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA149750.12133289062020189Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC146690.11885356744625986Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144910.11741134677645045No Hit
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCC136310.11044331432680948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTAAGTGGGAAACGATGTGGGAAGGAGATCGGAAGAGCACACGTCTGA135030.10940621182267687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC132520.10737251863097932Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC127990.1037021480499475Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)