FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15185556
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG162334910.690086026484641TruSeq Adapter, Index 7 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG2449501.6130459760577749No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1603911.0562076225592267Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA513440.3381107678902241Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC387470.2551569399237012Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT372510.24530547317464044No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA331470.21827979166518502Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC330670.21775297526149193Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG325390.2142759869971175No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG316360.20832954684043176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA309220.20362771043747097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG272450.1794139114827274TruSeq Adapter, Index 7 (100% over 36bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC260290.17140630214659247Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC240190.15817004000380364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC227700.14994511890114526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC207110.1363861817110944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201280.13254700716918102No Hit
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC188640.12422330799083023Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG187500.12347259461556759TruSeq Adapter, Index 7 (100% over 36bp)
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA187320.12335406092473664TruSeq Adapter, Index 7 (100% over 35bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT172480.11358161663622984TruSeq Adapter, Index 7 (100% over 48bp)
CGTGTTCGGAAGATTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC171900.11319967474355236Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG169150.11138874335585736Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC160120.10544230319917164TruSeq Adapter, Index 7 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC154350.10164263988753525No Hit
AGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG153280.10093802294759574TruSeq Adapter, Index 7 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)