FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20369832
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG371820818.253503514412884No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG13846766.797680020139587TruSeq Adapter, Index 9 (100% over 49bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA7753623.806423145757903Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG4021151.9740712638179831No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC3265711.603209098631741No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT2484561.219725327140646No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1727770.8482004171659344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG1618640.7946260921543192No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1526270.7492796209610368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1395460.6850621055686665Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC1350340.6629117019718179Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC1345310.660442363982187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1298900.637658670920801Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1260420.6187679898390914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1241650.6095533826690372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1231290.6044674300701155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC1103150.5415606765927181Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA958940.4707648055222056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC912610.44802038622606216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA901350.4424926037681607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA772180.37908020056326436No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC657880.32296780847284356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA548920.26947694021236895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTC462490.22704654608835265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC425720.20899534173870457No Hit
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC417610.20501396378723202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG359710.17658957619287188Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC357460.17548500154542268No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC347670.1706788745238547No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG313540.15392370442721373No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT309910.152141657329329TruSeq Adapter, Index 9 (100% over 36bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT294320.14448818232766966TruSeq Adapter, Index 9 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC287770.14127264279842858No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA277030.13600013981460427Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA275150.13507720633140224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT273250.13414445440688957Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC269620.1323624073090048Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC249070.12227395886230186Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC247610.12155721264662368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC243620.11959843360514708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC228770.11230824093198216Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT225940.1109189314865238Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC213380.10475295034342944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC211690.1039232920526787Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC209710.10295126636292336Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)