FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11047457
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG112797010.210223040469856TruSeq Adapter, Index 10 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG7654196.928463265346949No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA3341583.024750401834558Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA1170601.0596103700607298Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG902710.8171201752584327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC729770.660577361830872No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC683490.6186853680444286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG612820.5547158952508257Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT506880.4588205231303457No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC464500.42045875353938916Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA444130.4020201210106543Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA391770.35462459822201614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC381740.34554558573977706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC378090.34224165796707784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG355960.3222098986219181No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC352380.3189693338475995Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC291060.26346334726625326Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG288640.2612727978936691Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG273900.2479303608061113TruSeq Adapter, Index 7 (97% over 36bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC248810.22521925181514624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC235660.21331605997651765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC218410.19770160680417223No Hit
AACTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT211800.19171832938566766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC203730.18441348085808346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC202420.18322768760267633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC192070.17385901569926906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA185490.16790289385149904No Hit
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA173250.1568234209918174Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG169350.1532931967963306TruSeq Adapter, Index 7 (97% over 36bp)
AGCTCTCAACAACGGATATCTTGGCTCTCAGATCGGAAGAGCACACGTCT152380.13793219561750727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC143590.12997561339229471No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC135710.12284275014602909Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT132890.12029012649698478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC127960.11582756103961302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC126270.11429779722156871Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115610.10464851775390481No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA114780.10389721363024992Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA110480.10000491515830294Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)