FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90481838
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG13232911.4624935006293749No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG10285291.136724256198244TruSeq Adapter, Index 11 (100% over 49bp)
CGGCCTGGTGGCCTGAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCC2477560.27381848719739754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2067140.2284591080035311Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAAC1966620.21734969618985855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1942580.21469280940115296Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1904570.21049196635461806TruSeq Adapter, Index 6 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1892510.20915910218357855No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1805930.19959033104522036TruSeq Adapter, Index 6 (97% over 36bp)
GTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACT1792230.19807621503002623Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGA1657540.18319035473174186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAA1610750.1780191512024767Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1593860.1761524782465184Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC1502410.16604547754655472TruSeq Adapter, Index 11 (100% over 42bp)
CGGCCTGGTGGCCTGAGCGGTAGATCGGAAGAGCACACGTCTGAACTCCA1469700.1624303874110073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTG1318570.14572758789448995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT1229380.1358703610773247TruSeq Adapter, Index 11 (100% over 41bp)
CCCGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCT1060530.11720915748860009Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT1034450.11432681108887288Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC1017030.11240156284181584No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT1016470.11233967196820206No Hit
CTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATC931520.10295104747982683TruSeq Adapter, Index 11 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)