FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11902564
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG121092710.173665102745929TruSeq Adapter, Index 12 (100% over 49bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3141892.639674947347479No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1190701.000372692808037No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCA653530.5490665708665796Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC630280.5295329644940368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG613620.5155359803148296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT571420.48008143455477326No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT521220.43790564789233644No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG515700.43326799166969404No Hit
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA488640.41053339431739244Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG415280.3488996152425645No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC391150.32862667237075976No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA299140.2513240004422576TruSeq Adapter, Index 12 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG242390.20364519779099693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC241430.20283864888271133Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG237310.19937720981798546Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA228290.19179901070055158Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT227890.19146294865543256TruSeq Adapter, Index 12 (100% over 36bp)
AGTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA221040.18570788613276937TruSeq Adapter, Index 12 (100% over 40bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC216000.18147350436426976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC208210.17492869603557687No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC202090.1697869467452559Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC200870.1687619575076429Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC186930.15705019523524513Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT185690.15600840289537615TruSeq Adapter, Index 12 (100% over 41bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG175000.1470271447395704No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT174950.1469851369839305No Hit
AGTTGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACT169260.1422046543921125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC168910.14191060010263337Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT160970.13523976850702085TruSeq Adapter, Index 12 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT157040.13193795891372648No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC156530.13150947980619976No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC152220.12788841127004233Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT147500.12392287913763791TruSeq Adapter, Index 12 (100% over 36bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC142750.11993214235184955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA142400.11963808806237043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139550.11724364599089741No Hit
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC135570.1138998286419632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCC129000.10838000955088332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG121250.10186880742670236No Hit
GTTGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTC120660.10137311591015179Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)