FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008061333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008061333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868056
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3315224.827013641123485No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG3097794.510432064036752TruSeq Adapter, Index 14 (97% over 49bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA693681.0100092369660352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG494400.7198543517991117No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC324790.4728994638366373No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT283640.41298440199089814No Hit
AGATTAGTGGAACGCTCTGGAAAGTGCAGATCGGAAGAGCACACGTCTGA242520.353113020627671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGATTAGTGGAACGCTCTGGAAAGTGCAGATCGGAAGAGCACACGTCTG216010.3145140342478279Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC208450.30350655265478327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG205980.2999101929279551No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA175720.25585114623410177Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC139930.2037403305971879Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC137390.2000420497444983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT137040.1995324441151907TruSeq Adapter, Index 8 (97% over 36bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC136730.1990810791292325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC119350.17377551959389964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT117770.17147501418159666TruSeq Adapter, Index 8 (97% over 36bp)
CGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA115980.1688687453917091Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG113890.1658256717767007Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA110380.16071505532278713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGATTAGTGGAACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA98320.14315550135293015Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGATTAGTGGAACGCTCTGGAAAGTGCAGATCGGAAGAGCACACGTCT96450.14043275127634372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC88000.12812941536877392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC86490.12593083108233247Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC86090.12534842464883805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGTTCGGAAGATTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC83080.12096581623679248Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC82660.12035428948162331Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC80780.11761697924419952No Hit
GAGTGACGTAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG80700.11750049795750063TruSeq Adapter, Index 2 (97% over 35bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC80030.11652496718139749Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC78690.11457390562919115TruSeq Adapter, Index 14 (100% over 39bp)
AGATTAGTGGAACGCTCTGGAAAGTGTAGATCGGAAGAGCACACGTCTGA73060.10637653507775707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC71730.10444003368638811Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC71220.10369746548368271Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG70870.10318785985437509Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATTAGTGGAACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC70760.10302769808516413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA69040.1005233504211381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)